Cut and Run analysis for Christopher Douse

This project looks at differential binding of wild type TASOR (D3 and WTT) and modified versions of this protein: a mutated version (muT) and a KO version (TKO).

Question: Are there any differences between D3 and WTT? If yes, how do they differ?

Samples have been generated with a modifed version of the Cut&Run protocol, published in and modified as described in Material and Methods of our paper (insert bioRxive link to paper). The Cut&Run protocol produced chip-seq like reads (albeit smaller and with a much better resolution) which we can then use to call in peaks and to call either presence and abscence or differential binding.

We implement the use of RUVseq to remove unwanted variation in the Chipseq data. This has been previously been done in .